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m mlv reverse transcriptase  (TaKaRa)


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    Structured Review

    TaKaRa m mlv reverse transcriptase
    M Mlv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+rt+kits/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/10__1016_slash_j__ejar__2026__09__002-67-0-4
    Average 99 stars, based on 15291 article reviews
    m mlv reverse transcriptase - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Aging Regulates Receptivity by Modulating the Expression of Osteopontin and HOXA10 in the Human Endometrium
    Article Snippet: Total RNA isolation was performed using TRIzol (15596026; Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions. .. One microgram of RNA was added for cDNA synthesis using the TAKARA (Kusatsu, Shiga, Japan; RR037A) RT cDNA synthesis kit. .. For semi-quantification of the genes of interest, real-time PCR was performed on an Mx300P cycler using the Universal qPCR kit (KK4602; KAPABiosystems, Cape Town, South Africa) and specific primers ( ) for each gene analyzed.

    Article Title: Enhanced carbon capture and storage in the green microalga Chlamydomonas by heterologous expression of an engineered carbonic anhydrase.
    Article Snippet: Microalgae are regarded as a promising platform for carbon capture and storage (CCS).. A key target for enhancing CCS in microalgae is the carbon-concentrating mechanism (CCM), which is heavily reliant on carbonic anhydrase (CA) activity.. Overexpressing CA in microalgae may offer an effective approach to capture excess CO2.

    Article Title: Hepatic IFRD1 suppresses metabolic dysfunction-associated fatty liver disease via GLUD1/α-KG axis
    Article Snippet: Journal Pre-proofs Article Hepatic IFRD1 suppresses metabolic dysfunction-associated fatty liver dis‐ ease via GLUD1/α-KG axis Mengya Geng, Fanzheng Meng, Hairui Li, Lijie Sun, Xuedan Sun, Yu Ding, Rongdongqing Shi, Zhihua Wang, Yabin Huang, Jizhou Wang, Yao Liu, Jiabei Wang, Peter J.. Little, Suowen Xu, Lianxin Liu, Jianping Weng, Sihui Luo PII: S2095-9273(26)00365-8 DOI: https://doi.org/10.1016/j.scib.2026.04.016 Reference: SCIB 3996 To appear in: Science Bulletin Received Date: 28 October 2025 Revised Date: 24 February 2026 Accepted Date: 3 April 2026 Please cite this article as: M. Geng, F. Meng, H. Li, L. Sun, X.. Sun, Y. Ding, R. Shi, Z. Wang, Y. Huang, J. Wang, Y. Liu, J. Wang, P.J.

    Reverse Transcription:

    Article Title: Enhanced carbon capture and storage in the green microalga Chlamydomonas by heterologous expression of an engineered carbonic anhydrase.
    Article Snippet: Microalgae are regarded as a promising platform for carbon capture and storage (CCS).. A key target for enhancing CCS in microalgae is the carbon-concentrating mechanism (CCM), which is heavily reliant on carbonic anhydrase (CA) activity.. Overexpressing CA in microalgae may offer an effective approach to capture excess CO2.

    Article Title: Melatonin Suppresses NLRP3 Inflammasome Activation via SIRT1-Mediated ETS1 Deacetylation to Attenuate LPS-Induced Pyroptosis in Alveolar Epithelial Cells
    Article Snippet: .. After chloroform extraction and isopropanol precipitation, RNA was reverse-transcribed into cDNA using a reverse transcriptase kit (Cat. No. #RR036A; Takara Ltd., Otsu, Japan). .. Quantitative PCR was performed using TB Green Premix Ex Taq II (Cat. No. #RR820A; Takara Ltd). on an ABI 7300 real-time PCR system (Applied Biosystems Inc).

    Article Title: Hepatic IFRD1 suppresses metabolic dysfunction-associated fatty liver disease via GLUD1/α-KG axis
    Article Snippet: Journal Pre-proofs Article Hepatic IFRD1 suppresses metabolic dysfunction-associated fatty liver dis‐ ease via GLUD1/α-KG axis Mengya Geng, Fanzheng Meng, Hairui Li, Lijie Sun, Xuedan Sun, Yu Ding, Rongdongqing Shi, Zhihua Wang, Yabin Huang, Jizhou Wang, Yao Liu, Jiabei Wang, Peter J.. Little, Suowen Xu, Lianxin Liu, Jianping Weng, Sihui Luo PII: S2095-9273(26)00365-8 DOI: https://doi.org/10.1016/j.scib.2026.04.016 Reference: SCIB 3996 To appear in: Science Bulletin Received Date: 28 October 2025 Revised Date: 24 February 2026 Accepted Date: 3 April 2026 Please cite this article as: M. Geng, F. Meng, H. Li, L. Sun, X.. Sun, Y. Ding, R. Shi, Z. Wang, Y. Huang, J. Wang, Y. Liu, J. Wang, P.J.

    Article Title: KRT15 identified by scRNA-Seq and machine learning as stemness regulator and prognostic biomarker in ESCC
    Article Snippet: .. M-MLV Reverse Transcriptase (RNase H-) Kit , Takara Bio , Cat# RR047A. .. TB Green Premix Ex Taq II , Takara Bio , Cat#RR820A.

    Article Title: FOSL1 Mediates Collagen Synthesis and Myofibroblast Transformation in Aortic Fibroblasts Under Ang II-Induced Aortic Dissection Conditions.
    Article Snippet: .. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was extracted from AFs using Trizol reagent (Ambion, 15596026) and reverse transcribed into Complementary DNA (cDNA) using a Prime Script reverse transcriptase reagent kit (Takara, RR037A). .. RT-qPCR analysis was performed using TB Green Premix Ex Taq (Takara, RR820A). β-actin served as an internal control for normalization.

    Article Title: Mutual feedback regulation between Poly(A)-specific ribonuclease (PARN) and cognate microRNAs.
    Article Snippet: RNA concentration was determined by spectrophotometry (NanoDrop 2000; Thermo Fisher Scientific). .. One microgram of total RNA was reverse-transcribed by Moloney murine leukemia virus (M-MuLV) reverse transcriptase (PrimeScript RT–PCR kit; Clontech) using oligo12–18 as the reverse transcription primer. cDNA equivalent to 20 ng of total RNA was subjected to qRT-PCR using specific primers for miRNA-29a, pri-miRNA-29a. ..

    Article Title: Mutual feedback regulation between Poly(A)-specific ribonuclease (PARN) and cognate microRNAs
    Article Snippet: RNA concentration was determined by spectrophotometry (NanoDrop 2000; Thermo Fisher Scientific). .. One microgram of total RNA was reverse-transcribed by Moloney murine leukemia virus (M-MuLV) reverse transcriptase (PrimeScript RT–PCR kit; Clontech) using oligo 12–18 as the reverse transcription primer. cDNA equivalent to 20 ng of total RNA was subjected to qRT-PCR using specific primers for miRNA-29a, pri-miRNA-29a. ..

    Extraction:

    Article Title: Melatonin Suppresses NLRP3 Inflammasome Activation via SIRT1-Mediated ETS1 Deacetylation to Attenuate LPS-Induced Pyroptosis in Alveolar Epithelial Cells
    Article Snippet: .. After chloroform extraction and isopropanol precipitation, RNA was reverse-transcribed into cDNA using a reverse transcriptase kit (Cat. No. #RR036A; Takara Ltd., Otsu, Japan). .. Quantitative PCR was performed using TB Green Premix Ex Taq II (Cat. No. #RR820A; Takara Ltd). on an ABI 7300 real-time PCR system (Applied Biosystems Inc).

    Real-time Polymerase Chain Reaction:

    Article Title: FOSL1 Mediates Collagen Synthesis and Myofibroblast Transformation in Aortic Fibroblasts Under Ang II-Induced Aortic Dissection Conditions.
    Article Snippet: .. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was extracted from AFs using Trizol reagent (Ambion, 15596026) and reverse transcribed into Complementary DNA (cDNA) using a Prime Script reverse transcriptase reagent kit (Takara, RR037A). .. RT-qPCR analysis was performed using TB Green Premix Ex Taq (Takara, RR820A). β-actin served as an internal control for normalization.

    Virus:

    Article Title: Mutual feedback regulation between Poly(A)-specific ribonuclease (PARN) and cognate microRNAs.
    Article Snippet: RNA concentration was determined by spectrophotometry (NanoDrop 2000; Thermo Fisher Scientific). .. One microgram of total RNA was reverse-transcribed by Moloney murine leukemia virus (M-MuLV) reverse transcriptase (PrimeScript RT–PCR kit; Clontech) using oligo12–18 as the reverse transcription primer. cDNA equivalent to 20 ng of total RNA was subjected to qRT-PCR using specific primers for miRNA-29a, pri-miRNA-29a. ..

    Article Title: Mutual feedback regulation between Poly(A)-specific ribonuclease (PARN) and cognate microRNAs
    Article Snippet: RNA concentration was determined by spectrophotometry (NanoDrop 2000; Thermo Fisher Scientific). .. One microgram of total RNA was reverse-transcribed by Moloney murine leukemia virus (M-MuLV) reverse transcriptase (PrimeScript RT–PCR kit; Clontech) using oligo 12–18 as the reverse transcription primer. cDNA equivalent to 20 ng of total RNA was subjected to qRT-PCR using specific primers for miRNA-29a, pri-miRNA-29a. ..

    Quantitative RT-PCR:

    Article Title: Mutual feedback regulation between Poly(A)-specific ribonuclease (PARN) and cognate microRNAs.
    Article Snippet: RNA concentration was determined by spectrophotometry (NanoDrop 2000; Thermo Fisher Scientific). .. One microgram of total RNA was reverse-transcribed by Moloney murine leukemia virus (M-MuLV) reverse transcriptase (PrimeScript RT–PCR kit; Clontech) using oligo12–18 as the reverse transcription primer. cDNA equivalent to 20 ng of total RNA was subjected to qRT-PCR using specific primers for miRNA-29a, pri-miRNA-29a. ..

    Article Title: Mutual feedback regulation between Poly(A)-specific ribonuclease (PARN) and cognate microRNAs
    Article Snippet: RNA concentration was determined by spectrophotometry (NanoDrop 2000; Thermo Fisher Scientific). .. One microgram of total RNA was reverse-transcribed by Moloney murine leukemia virus (M-MuLV) reverse transcriptase (PrimeScript RT–PCR kit; Clontech) using oligo 12–18 as the reverse transcription primer. cDNA equivalent to 20 ng of total RNA was subjected to qRT-PCR using specific primers for miRNA-29a, pri-miRNA-29a. ..



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